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Methanogenic archaea are important contributors to the Earth’s anaerobic carbon cycle, but their regulatory mechanisms remain poorly understood. This is particularly true for archaeal F-ATPases. To gain insight into the F-ATPase-regulatory mechanisms in archaea, we used 2-D PAGE and MS to investigate the effects of mutation of the F-ATPase subunit sdaV in Methanosarcina mazei. The proteins involved in glycolysis, TCA cycle, and protein synthesis were dramatically altered in sdaV mutants. Among them, phosphoenolpyruvate carboxykinase (PEPCK) and aspartate-semialdehyde dehydrogenase (ASADH) were present at higher levels in sdaV mutants, suggesting that the pathways of glycolysis and the TCA cycle may be shifted. Furthermore, tricarboxylic acid cycle (TCA) enzymes and subunits of the F-ATPase and F1-sector were differentially altered in the sdaV mutants compared with the wild-type strain. The altered proteins included glycolytic enzymes, TCA cycle enzymes, and F-ATPase F1 subunits. Interestingly, the F-ATPase F1 beta subunit and its assembling factor DnaK were also found to be severely decreased in sdaV mutants. Our study provides, to our knowledge, the first evidence demonstrating that the F-ATPase was required for the expression of the F1-sector in archaea.Fusiform lissencephaly: MRI and CT studies.
Fusiform lissencephaly is a very rare disorder caused by defects in migration and proliferation of the progenitors of the neural stem cells. We present a case of fusiform lissencephaly with fluid attenuated inversion recovery (FLAIR) hyperintensity on magnetic resonance imaging. The FLAIR hyperintensity was the result of partial preservation of the deep brain nuclei. Also, the coronal section

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