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The detection of Ras mutations in cytological materials from lung tumors is a promising diagnostic tool, although attempts to implement it in routine clinical practice have so far failed. Moreover, the potential role of KRAS mutations as independent prognostic and predictive factors in lung cancer is still controversial. The aim of this study was to test the diagnostic sensitivity and specificity of the KRAS mutation detection in cytological materials in comparison with surgically resected tissue samples. Material from 40 NSLCL patients (cases) with a single or dominant lung tumor site and histopathologically confirmed malignancy were collected. The tumor tissue and cell blocks were additionally tested for KRAS mutations. The specimens were analyzed by direct sequencing. KRAS mutations in the tumor tissue were detected in 14 of 40 patients (35%). These results were confirmed by the results obtained from the cell blocks. However, 16 of the 40 patients (40%) had a KRAS mutation in the tumor tissue and corresponding cell blocks. In 10 patients, KRAS mutations were detected in both the tumor tissue and the corresponding cell block, in 4 patients in the cell block only, and in 2 patients in the tumor tissue only. It was suggested that the frequency of KRAS mutations in the tumor tissue was significantly higher than that of the corresponding cell block. The results suggest that KRAS mutations cannot be reliably detected in cytological materials, although a number of mutations can be detected in cell blocks obtained from cell blocks.

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