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Category:Urdu-language literature
Category:Islamic terminology
Category:Pakistani literatureModeling of multinucleate myocardial regeneration in the mouse.
We have developed a three-dimensional model of myocardial regeneration capable of investigating the role of multinucleate myocardial cell clusters in the context of the complex interaction between cell proliferation, maturation, and hypertrophy. The model is based on computer-controlled stereolithography, which is an additive manufacturing technology that precisely fabricates three-dimensional solid objects. This method enabled us to create computerized models with several anatomical complexities. These models reproduce the natural branching architecture of the compact myocardium in the mouse. The ability to create multicellular layers 3-4 cm long with vessel-sized interconnecting channels allowed us to precisely study the interaction between different cell types. Multinucleate myocardial cell clusters (MNCs) with up to 12 nuclei were generated by means of vascular adhesion techniques. In situ hybridization and immunocytochemical staining for the presence of myocyte enhancer factor 2c and for the mitotic marker Ki67 were performed on myocardial samples generated at 2 and 7 days after adhesion. The multinucleate myocardial cell cluster model provides a reproducible 3-D in vitro environment that offers a unique tool for investigating the molecular and cellular mechanisms of multinucleate myocardial cell clusters.Stress-induced adaptation: peripheral blood lymphocyte superoxide generation and level of corticosterone secretion in mice.
We studied stress-induced peripheral blood lymphocyte superoxide generation and the level of corticosterone secretion in mice (Balb/c mice, Dark-type (Dt) mice and Agouti-type (A(y)) mice). Peripheral blood lymphocytes were isolated by the method of Eyskens et al., and cultured in RPMI 1640 medium for 2 h. After incubation, superoxide generation was measured by the method of Hibino et al. using a NBT assay. The level of serum corticosterone was measured by the method of Ichinose et al. from 3-h blood samples taken from mice after an ether anesthesia. Superoxide generation was increased in a time-dependent manner in dark-type mice after the ether anesthesia, while it was not increased in Agouti-type mice. This transient increase in superoxide generation in dark-type mice was suppressed by n
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