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This study aims to investigate the effects of u-50,488h (a kappa opioid agonist) on resiniferatoxin (RTX)-induced heat hyperalgesia and allodynia, and to evaluate the possible involvement of spinal NMDA receptor subunit 1 (NR1) in u-50,488h-mediated analgesia in rats. In the behavioral studies, the time course of heat-induced allodynia and hyperalgesia were evaluated in response to a radiant heat stimulus. In the study of the actions of u-50,488h, the effects of u-50,488h were examined on RTX-induced heat hyperalgesia and allodynia, in rats. In the first experiment, rats were treated with RTX (30 nmol; 0.5 microL, intrathecally). Three days later, we investigated the acute effect of u-50,488h (50-500 nmol, intraperitoneally, i.p.) on RTX-induced thermal hyperalgesia, hyperalgesia, and allodynia. In the second experiment, we examined the effects of intraperitoneal (i.p.) administration of u-50,488h, on RTX-induced thermal hyperalgesia and allodynia. The presence of thermal allodynia was assessed by withdrawal latency measurements in response to a radiant heat stimulus (50-200 mW). In the experiment assessing thermal hyperalgesia, latency was measured in response to a radiant heat stimulus that produced the nociceptive response in healthy animals. Moreover, the effects of u-50,488h on the

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Batman:Arkham City DLC [lossless Repack] By R.G.Catalyst Money Hack

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