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The arrest of Colonel Zakharchenko: all the details and photos
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The arrest of Colonel Zakharchenko: all the details and photos
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The Gluconobacter strain JCM7456 has three genes (ognd, opxA and opcAB) encoding a bifunctional protein, a constitutively expressed oxygenase and a transcriptional repressor, respectively. In order to study the synergistic effect of oxygenase-deficient genes on production of 1,3-propanediol from glycerol, an ognd deletion mutant was isolated from JCM7456. The mutant strain, JCM7451, had about 40% loss of the oxygenase activity, but also had about 10-fold increase in the glycerol dehydratase activity and higher efficiency of converting glycerol to 1,3-propanediol under aerobic conditions as compared to JCM7456. The current results also showed that JCM7451 strain could produce 1,3-propanediol in the absence of oxygen. The JCM7451 strain required more glucose for growth and exhibited enhanced biomass accumulation than the JCM7456. The yield of 1,3-propanediol was 0.41 g/g when JCM7456 was cultivated in a 2.5 L fermentor for 22 h and 0.5 g/g when JCM7451 was cultivated in a 5.0 L fermentor for about 110 h.A method of spatial radiation exposure assessment according to the U.S. Society of Photogrammetry and Remote Sensing and the European Photogrammetric and Remote Sensing Association (
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