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Flama Condensed Font Released by Mario Feliciano. A design inspired by the typography of .Continuous delivery of a genetically modified nanoparticle vaccine against tuberculosis.
Disseminated Mycobacterium tuberculosis (Mtb) infection is a major world public health problem and the leading cause of human death due to a single infectious agent. An attractive strategy to combat this disease is the delivery of antigen presenting cells (APCs) to lymphoid tissues by means of an immunostimulatory molecular adjuvant. The efficacy of nanoparticle vaccination with DNA sequences encoding APC cell-associated receptors could be significantly enhanced by linking the vaccine to a cationic liposome. This approach leads to efficient targeting of the APCs to the lymphoid tissues, which ultimately enhances the immunogenicity of the vaccine. In this study we developed an in vitro model to evaluate the immune response of human monocytes to the vaccine, which was achieved by genetic modification of these cells with the recombinant vaccinia virus, Leiger. This approach allowed us to demonstrate that Leiger-infected human monocytes can be efficiently targeted to lymph nodes by means of an in vitro immunisation strategy and their activation to become APCs could be achieved by the addition of specific Toll-like receptor (TLR) 4 ligands. Our data demonstrate that cationic liposome-Mtb-DNA nanoparticles are potent stimulators of human dendritic cells (DCs). An evaluation of the mechanisms of DC activation by the vaccine showed that DCs co-treated with these TLR ligands and cationic liposome-Mtb-DNA nanoparticles exhibited greater levels of activation than cells treated with the vaccine alone. Importantly, cationic liposome-Mtb-DNA nanoparticles and Leiger-infected monocytes were able to stimulate production of antigen-specific interferon-gamma (IFN-gamma) and interleukin-2 (IL-2). These results point to the potential use of Leiger-infected monocytes as a carrier for the delivery of vaccine-encoding DNA sequences to the APCs of the lymphoid tissues.// Copyright 2009 the Sputnik authors. All rights reserved.
// This code is governed by the BSD license found in the LICENSE file.

/**
* @name: S15.7.3_A1_T1;
* @section: 15.
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