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The complete mitochondrial genome of Leishmania (Viannia) braziliensis was amplified from a single promastigote DNA-containing sample by PCR. Twenty-four overlapping cDNA fragments were generated by RT-PCR of RNA from the same sample and sequenced. Through sequencing of cDNA-PCR products, the sequence of 29 Kb of the genome was completed, generating the largest mitochondrial genome described for any eukaryote to date. The mitochondrial genome consists of two large (>10 Kb) and a small (2 Kb) circular DNA molecules. The mitochondrial gene arrangement was revealed through a comparison of the sequence data obtained from the two large DNA molecules with the mitochondrial genome of Leishmania tarentolae. The L. (V.) braziliensis sequence data was found to contain sequences identical to the corresponding genome of Leishmania tarentolae, such as the displacement loop, most tRNAs, three regions of rRNA genes and the ribosomal proteins. The 5′ end of the large DNA molecule contained all of the elements that have been previously shown to be associated with the endonuclease gene necessary for mitochondrial chromosome circularization and the nuclear-encoded tRNA-Arg. Southern blot analysis of DNA from different life-stages of the parasite, including vectors and hosts, revealed the presence of a single major mtDNA molecule (approximately 20 Kb) in all life-stages. The PCR generated mtDNA molecules were cloned into Escherichia coli and a Southern blot analysis confirmed that the cloned mtDNA molecules were free of the nuclear genome and exhibited the expected size.Last updated on.From the section Rugby Union

Tommy Bowe has won 84 caps for Ulster and Ireland

Ulster have signed Munster’s Tomaweesi Tupou after the IRFU ruled he had played out of round with Wales.

The 24-year-old South African lock is available for the start of Ulster’s 2019-20 Championship campaign.

Tupou played in the second and third rounds of the competition, having been left out of Munster’s 26-13 defeat by Zebre on 23 March.

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