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the objective of this chapter is to show the constituents and tests for microbial aerosols. such aerosols were identified in the air during the space station mir mission on june 18, 1994. the composition of microbial aerosols is quite different from the composition of particles in the conventional environmental air. the microbial aerosols contain bacteria, viruses, and a wide range of micro- and macroorganisms.
we propose that a microfluidic interface developed for our lab permits detection of bacterial activity of flow-through cell culture systems. the microfluidic device is composed of a pdms pate base with an array of microchannels sandwiched between two polycarbonate slides. a set of flow-cell chambers is installed at the bottoms of the microchannels. a bilayered agar medium is accommodated in the cell chambers to keep each chamber separate from adjacent chambers. stagnant liquid is drained out of the sample chambers by the connecting microchannels.
the samples were collected in duplicate, summing to 10 sub– samples of 100 ml each. each sample corresponded to a mix of 10 sub– samples, composed of 10-ml aliquots, collected along one linear meter. immediately after collection the samples were homogenized in 100-ml sterilized flasks and kept at 4c tested in the microbiology laboratory of the health sciences center at unisinos. the samples were collected in two sites, one at the irrigation channel (ic) and the other at the rice-field plot (rp). two collections were made throughout the rice growing cycle. the first one (p1) was obtained 15 days after the plots’ irrigation, between november 2001 and february 2002 and the second one (p2) was obtained close to harvesting or draining of plots, and finished by the end of april 2002.
the water samples were collected between november 2001 and april 2002 in the five rice crop regions of rs. they were collected in duplicates, summing 10 sub- samples of 100 ml each. each sample corresponded to a mix of 10 sub- samples, composed of 10-ml aliquots, collected along one linear meter. immediately after collection the samples were homogenized in 100-ml sterilized flasks and kept at 4c tested in the microbiology laboratory of the health sciences center at unisinos. the samples were collected in two sites, one at the irrigation channel (ic) and the other at the rice-field plot (rp). two collections were made throughout the rice growing cycle. the first one (p1) was obtained 15 days after the plots’ irrigation, between november 2001 and february 2002 and the second one (p2) was obtainedclose to harvesting or draining of plots, and finished by the end of april 2002.
the samples were collected in duplicate, summing to 10 sub- samples of 100 ml each. each sample corresponded to a mix of 10 sub- samples, composed of 10-ml aliquots, collected along one linear meter. immediately after collection the samples were homogenized in 100-ml sterilized flasks and kept at 4c tested in the microbiology laboratory of the health sciences center at unisinos. the samples were collected in two sites, one at the irrigation channel (ic) and the other at the rice-field plot (rp). two collections were made throughout the rice growing cycle. the first one (p1) was obtained 15 days after the plots’ irrigation, between november 2001 and february 2002 and the second one (p2) was obtainedclose to harvesting or draining of plots, and finished by the end of april 2002.
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