‘Pioneer Hill Software SpectraPLUS 5.0.26.0’ \/\/FREE\\\\

‘Pioneer Hill Software SpectraPLUS 5.0.26.0’ \/\/FREE\\\\

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‘Pioneer Hill Software SpectraPLUS 5.0.26.0’

rat vocalizations were recorded from a home cage over a period of 10 days with a 1/4-inch condenser microphone (type4158n, detection range 20100khz; aco co., ltd., tokyo, japan) in a recording chamber of 35cm (d)40cm (w)30cm (h) dimensions (ohara and co., tokyo, japan). the microphone was placed 30cm above the floor. the voice signals were transformed using a 192-khz ad convertor (spectradaq-200, aco co.), processed by an on-time fast fourier transform (fft) analyzer (spectraplus-sc; pioneer hill software llc, poulsbo, wa, usa), and recorded in the wav format. the sound files were further analyzed off-line using the same software with a hanning window, an fft size of 8192 (time resolution, 21.33ms; frequency resolution, 23.438hz), an fft overlap of 50%, an amplitude span of 60db, and a plot range of 65db. the numbers of rat vocalizations were counted manually in the frequency ranges of 1030khz (i.e., low pitch) and 3080khz (i., high pitch). alternatively, the sound files were analyzed for figure display by the raven pro 1.6.1 program (the cornell lab of ornithology, ithaca, ny, usa) with a hanning window, an fft size of 512 (time resolution, 2.7ms; frequency resolution, 375hz), and an fft overlap of 50% (center for conservation bioacoustics, 2019).

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