Pulpfiction1994hindidubbedddl

Pulpfiction1994hindidubbedddl


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Any other info you would like to share? Please make sure to leave it in the comments!Distinct role for the differential expression of human metallothionein genes in cell differentiation and responses to oxidative stress.
In mammals, six isoforms of metallothionein (MT), a family of low molecular weight proteins containing metal binding domains, are expressed. MT is most notably known for its role in tolerance to metal exposure and heavy metal detoxification, but recent studies have linked MT to carcinogenesis and stress responses. MT is known to be differentially regulated in a number of cell systems, including human cell lines, tissues and tumors. Although transcriptional regulation of MT has been well-characterized, the role of post-transcriptional regulation of human MT remains largely unstudied. To determine the effect of differential MT expression on cell phenotype, we have used a human MT-IIA complementary DNA (cDNA) construct, transfected into human kidney cancer cells, to directly compare the response to oxidative stress of clones with high, intermediate and low levels of expression of MT-IIA. We found that clones with high and intermediate levels of MT-IIA expression demonstrated a phenotype distinct from MT-negative clones, whereas expression of MT-IIA did not significantly alter sensitivity to the oxidant, menadione. To determine whether differential MT expression is involved in the control of cell differentiation, we have also transfected MT-IIA into the human myeloid leukemia cell line, HL60, a cell line with well-documented induction to differentiate to myeloid cells in response to PMA (phorbol myristate acetate). We found that the MT-negative, differentiated phenotype could be modulated by transfection with MT-IIA cDNA. Taken together, these data suggest that the differential expression of MT can regulate cell differentiation as well as response to oxidative stress in a cell-specific manner.Q:

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On November 11, 2003, Pulp Fiction was named Best Film in the BAFTA British Academy Film Awards, the first time that film won an Academy Award.

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