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Emulsion stability of liquid quaternized ethoxylated cetyltrimethylammonium bromide as water/glycerol-based vehicle for hyaluronic acid (HA) at 20 degrees C was investigated with sonication and used to evaluate effect of HA on stability of the emulsion. To determine if charge neutralization by HA would improve stability of the emulsion, the liquid quaternized emulsion was prepared by addition of HA or water at 20 degrees C. Unsonicated emulsions of HA (1-20 mg/mL) were also prepared, and emulsion stability was assessed using sonication or heating at 60 degrees C for 10 minutes. When HA was added in the liquid quaternized emulsion, sonication stability was increased from a few minutes to up to several hours. The mechanism of stability increase likely involved charge neutralization by HA as the viscosity of the emulsion did not change significantly. When HA-free liquid quaternized emulsion was prepared, sonication stability was still very short. However, heating to 60 degrees C for 10 minutes significantly improved sonication stability. The results indicate that the emulsions containing HA are less stable than that without HA. We propose that the charge-neutralizing ability of HA reduces the negative surface potential of the liquid quaternized emulsion, thereby increasing the repulsive force between particles in the emulsion.Gene therapy for neural diseases.
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