UrbanismoBioclimaticoEsterHigueraspdf 🎇

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UrbanismoBioclimaticoEsterHigueraspdf

Ester Higueras sistematiza en un libro el conocimiento de la naturaleza y de la arquitectura para poner el territorio en red.

Category:Spanish architects
Category:Living people
Category:Year of birth missing (living people), although it is recommended that the probes be exposed to tissues for more than 15 minutes.

We express our special thanks to Nikolay Fedyanin and Alexander Kuznetsov for their assistance in conducting quantitative PCR experiments. The work was supported by the grant from the Russian Foundation for Basic Research (14-04-00616-a) and Moscow State University competitiveness improvement project (6315945094).

Conceived and designed the study: E.A.P. Performed the study: E.A.P. Contributed essential reagents or tools: E.A.P. Data analysis: E.A.P. and A.A.E. Wrote the paper: E.A.P., A.A.E. and V.N.K.

The authors declare no conflict of interest.

![(**A**) Schematic diagram of the kinetics of fluorescence based detection of PCR products. Excitation of SYBR Green I in fluorescent dye solution (green) is caused by light at the wavelength of 480 nm and emission of fluorescence at 520 nm; (**B**) Effect of amplified DNA concentration on fluorescence intensity. Note: the region of linear response is from 0.25–3.0 ng of DNA per reaction; (**C**) Dynamic range of fluorescence detected from 0.25 ng–1.0 ng of template DNA in qPCR using the method of mixed primers (one step) and pyrosequencing, compared with results obtained using two-step method (three-step method); (**D**) Results of pyrosequencing versus one-step method. A(4): C(5); D(2): E(2); F(2): H(3); G(1): K(4); and H(1): L(1); N(1): O(1); P(1): R(1).](ijms-14-00256f1){#f1-ijms-14-00256}

![(**A**) Gene mapping of *N*-nitrosopyrrol

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